Cloning and analysis of the human S13 ribosomal protein cDNA
نویسندگان
چکیده
منابع مشابه
cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca).
The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherichia coli BL21. We compared the nucleotide sequences of the coding regi...
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One of the many complex organelles in living organisms is the ribosome that catalyzes peptide bond formation during protein synthesis. Ribosomes contain two subunits and are ribonucleoprotein complexes composed of more than 70 r p s and four ribosomal RNA species (Wittman, 1982). Most of the rps are present in equimolar amounts, and expression of rp genes is coordinately regulated (Freid and Wa...
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15 صفحه اولSequence of a cDNA encoding human ribosomal protein L26 and of a cDNA probably encoding human ribosomal protein L6.
Mammalian ribosomes consist of 82 different proteins and four RNA molecules. Sequence analysis of ribosomal proteins is essential for structural and functional studies and for understanding the evolution of ribosomes (1). In a search for genes related to non-P-glycoprotein mediated multidrug resistance in human lung cancer cell lines (2), we isolated three full-length cDNA clones corresponding ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.12.2945